Biography
Curriculum Vitae
Daochun Kong
Degrees B.S. Lanzhou University, 1983
M.S. Shanghai Institute of Biochemistry, Chinese Academy of Science, 1989
Ph.D. Temple University Medical School, USA. 1995
Professional Training
1979-1983 Lanzhou University
1986-1989 Graduate Student, Shanghai Institute of Biochemistry, Chinese Academy of Science
1990-1995 Graduate Student, Department of Biochemistry, Temple University Medical School
1995-1998 Postdoctoral Fellow, Department of Biological Chemistry and Molecular
Pharmacology, Harvard Medical School
1998-2000 Postdoctoral Fellow, The Memorial Sloan-Kettering Cancer Center
Employment
1983-1986 Research Fellow, Lanzhou Institute of Biomedicine, China
1989-1990 Head, The Group of Protein Purification, Shanghai Academy of Biomedicine, China
Academic Appointments
2000-2005 Research scientist, National Institutes of Health, U.S.A
2005- Professor, The National Laboratory of Protein Engineering and Plant Genetic
Engineering, The College of Life Sciences, Peking University, China
2012- Investigator, Peking-Tsinghua Center for Life Sciences
Education
Professional Training
1979-1983 Bachelor, Lanzhou University
1986-1989 Master, Graduate Student, Shanghai Institute of Biochemistry, Chinese Academy
of Science
1990-1995 Doctor, Graduate Student, Department of Biochemistry, Temple University
Medical School
1995-1998 Postdoctoral Fellow, Department of Biological Chemistry and Molecular
Pharmacology, Harvard Medical School
1998-2000 Postdoctoral Fellow, The Memorial Sloan-Kettering Cancer Center
Social Services
Organizer for CSHAsia meetings of DNA Metabolism, Genomic Stability and Disease and Yeast and Life Sciences
Representative Peer-Reviewed Publications
1. Xizhou Li, Lu Wang, Xiaoqin Liu, Zeqi Zheng, Daochun Kong§. Cellular regulation and stability of DNA replication forks in eukaryotic cells. DNA Repair (Amst). 2022 Dec;120:103418. doi: 10.1016/j.dnarep.2022.103418. Epub 2022 Oct 10.
2. Sijie Liu, Xizhou Li, Xiaoqin Liu, Jingna Wang, Lingyan Li, Daochun Kong§. RNA polymerase III directly participates in DNA homologous recombination. Trends Cell Biol. 2022 Dec;32(12):988-995. doi: 10.1016/j.tcb.2022.06.007. Epub 2022 Jul 8.
3. Zizhi Tang, Ming Zeng, Xiaojun Wang, Chang Guo, Peng Yue, Xiaohu Zhang, Huiqiang Lou, Jun Chen, Dezhi Mu, Daochun Kong, Antony M Carr, Cong Liu. Synthetic lethality between TP53 and ENDOD1. Nat Commun. 2022 May 23;13(1):2861. doi: 10.1038/s41467-022-30311-w.
4. Sijie Liu & Daochun Kong§. A direct role of RNA polymerase III and RNA in DNA homologous recombination. Molecular & Cellular Oncology. 2021 Jul 1;DOI:10.1080/23723556.2021.1935173.
5. Sijie Liu, Xiaoqin Liu, Daochun Kong§. A Recently Discovered Essential Factor for DNA Homologous Recombination: RNA Polymerase III. Journal of Cell Science & Therapy. 2021 Jun 22;12(6):302.
6. Yang Liu#, Lu Wang#, Xin Xu, Yue Yuan, Bo Zhang, Zeyang Li, Yuchen Xie, Rui Yan, Zeqi Zheng, Jianguo Ji, Johanne M Murray, Antony M Carr, Daochun Kong§. The intra-S phase checkpoint directly regulates replication elongation to preserve the integrity of stalled replisomes. Proc Natl Acad Sci U S A. 2021 Jun 15;118(24):e2019183118.
7. Sijie Liu#, Yu Hua#, Jingan Wang#, Lingyan Li#, Junjie Yuan, Bo Zhang, Ziyang Wang, Jianguo Ji, Daochun Kong§. RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination. Cell. 2021 Mar 4;184(5):1314-1329.e10. doi: 10.1016/j.cell.2021.01.048. Epub 2021 Feb 23.
8. Lihong Wu, Yu Hua, Feiran Chang, Daochun Kong§. Girdin: an essential component of pre-replicative complex in human cells. BioRxiv. doi: https://doi.org/10.1101/634626 Posted 2019.5.13
9. Gang Feng#, Yue Yuan#, Zeyang Li, Lu Wang, Bo Zhang, Jiechen Luo, Jianguo Ji, Daochun Kong§. Replication fork stalling elicits chromatin compaction for the stability of stalling replication forks. Proc Natl Acad Sci U S A. 2019 Jul 16;116(29):14563-14572. doi: 10.1073/pnas.1821475116. Epub 2019 Jul 1.
10. Fanli Zeng#, Yu Hua#, Xiaoqin Liu, Sijie Liu, Kejing Lao, Ze Zhang, Daochun Kong§. Gpn2 and Rba50 Directly Participate in the Assembly of the Rpb3 Subcomplex in the Biogenesis of RNA Polymerase II. Mol Cell Biol. 2018 Jun 14;38(13). pii: e00091-18. doi: 10.1128/MCB.00091-18. Print 2018 Jul 1.
11. Ling Guan*, Peng He*, Fang Yang*, Yuan Zhang*, Yunfei Hu*, Jienv Ding*, Yu Hua, Yi Zhang,Qiong Ye, Jiazhi Hu, Tao Wang§, Changwen Jin§, Daochun Kong§, Sap1 is a replication initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe. J Biol Chem. 2017 Apr 14;292(15):6056-6075. doi: 10.1074/jbc.M116.767806. Epub 2017 Feb 21.
12. Bochao Liu, Jiazhi Hu, Jingna Wang, Daochun Kong§, Direct Visualization of RNA-DNA Primer Removal from Okazaki Fragments Provides Support for Flap Cleavage and Exonucleolytic Pathways in Eukaryotic Cells. J Biol Chem. 2017 Mar 24;292(12):4777-4788. doi: 10.1074/jbc.M116.758599. Epub 2017 Feb 3.
13. Huimin Zhang, Yu Hua, Rui Li, Daochun Kong§, Cdc24 Is Essential for Long-range End Resection in the Repair of Double-stranded DNA Breaks. J Biol Chem. 2016 Nov 25;291(48):24961-24973.
14. Ming Zeng, Laifeng Ren, Ken`Ichi Mizuno, Konstantinos Nestoras, Haibin Wang, Zizhi Tang, Liandi Guo, Daochun Kong, Qiwen Hu, Qun He, Lilin Du, Antony M. Carr, and Cong Liu, CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection. Nat Commun. 2016 Apr 21;7:11364.
15. Lihong Wu, Yang Liu, Daochun Kong§, Mechanism of chromosomal DNA replication initiation and replication fork stabilization in eukaryotes. Sci China Life Sci. 2014 May;57(5):482-7. Review.
16. Jiazhi Hu, Lei Sun, Fenfen Shen, Yufei Chen, Yu Hua, Yang Liu, Mian Zhang, Yiren Hu, Qingsong Wang, Wei Xu, Fei Sun, Jianguo Ji, Johanne M. Murray, Antony M. Carr, and Daochun Kong§, The intra-S phase checkpoint targets Dna2 to prevent stalled replication forks from reversing. Cell, 2012 , 149(6):1221-32.
Laboratory Introduction
